河北快3

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三维基因

sanweijiyinzuxue(three-dimensional genomics,3d genomics), jishizhizaikaolvjiyinzuxulie、jiyinjiegoujiqidiaokongyuanjiandetongshi,duiqizaixibaoheneidesanweikongjianjiegou,jizaijiyinzhuanlu、diaokong、fuzhihexiufudengshengwuguochengzhongdegongnengjinxingyanjiu。 weileyanjiujiyinzudesanweijiegou,meiguojob dekkerjiqihezuozheyu2002nianzuixiankaifachuransezhigouxiangbuhuo(chromatin conformation capture,3c)jishu。3cjishuyongyucedingyizhideliangduanjiyinxulie(dandianyudandian)zhijianshifoucunzaijiaohuzuoyong(xianxingjuliyuandeliangduanxulieyouyukongjianzhedieerbiandebicilinjin)。zhihou,kexuejiamenkuozhanle3cjishu,kaifale4c(circularized chromatin conformation capture)jishu,yongyucedingdandianyuduodianzhijianderansezhijiaohuzuoyong。5c(carbon-copy chromatin conformation capture)jishuyongyucedingduodianyuduodianzhijiandejiaohuzuoyong。weilenengbuhuoquanjiyinzufanweineisuoyouderansezhihuzuoxinxi,job dekkeryanjiutuanduiyu2009niankaifalegaotongliangransetigouxiangbuhuo(high-throughput chromosome conformation capture,hi-c)jishu。ciwai,jiangransetigouxiangbuhuojishuyumianyigongchendianjishuxiangjiehe,renmenkaifalechip-loop、chia-petdengjishu,yongyuyanjiutedingdanbaijiedaodejiyinjianjiaohuzuoyong。

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